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In a community-based longitudinal study of 3103 Chinese older adults free of MCI at baseline, Chen et al

GPX was measured adding cell extract to 50 mmol/l potassium phosphate pH 7.0 containing 1 mM EDTA, 1.5 mM sodium azide, 0.15 IU of purified glutathione reductase from bakers' yeast, 0.45 mM GSH, 0.2 mM NADPH, 1.2 mM cumene hydroperoxide One unit of GPX was defined as the amount of enzyme which produces 1 mol of GSSG (oxidized glutathione)/min at 25C

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